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The Bureau of Prohibition (or Prohibition Unit) was the federal law enforcement agency formed to enforce the National Prohibition Act of 1919, commonly known as the Volstead Act, which backed up the 18th Amendment to the United States Constitution regarding the prohibition of the manufacture, sale, and transportation of alcoholic beverages. When it was first established in 1920, it was a unit of the Bureau of Internal Revenue.

Its investigators were called Prohibition Agents, or more colloquially prohis.

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